The purpose of this study is to estimate the prevalence of Epstein Barr virus and Human Papilloma virus in salivary gland tumors. For this study 17 subjects (8 males, 9 females)
diagnosed as salivary gland tumors (11 cases of pleomorphic adenoma, 2 cases of adenoid cystic carcinoma, 2 cases of clear cell carcinoma, 2 mucoepidermoid tumor) referred to Dept. of Oral Pathology, College of Dentistry, Chosun University were used. For the detection of EBV (EBNA gene, BNRF-1 gene), and HPV (type 6,11,16,18) DNA was extracted from paraffin embedded specimens. By the PCR method, EBV DNA and HPV DNA were amplified through 30 cycles of denaturation, annealing and extension. After completion of the PCR, the solution was analyzed by 2% agarose gel electrophoresis. The gel was stained with ethidium bromide and examined under the ultraviolet transilluminator.
Obtained results are as follow,
1. HPV DNA was not detected in all the subjects.
2. EBNA gene was not detected in all the subjects.
3. BNRF-1 gene was detected in 14 cases (82.35%) out of 17cases.
4. BNRF-1 gene was detected in 10 cases of pleomorphic adenoma(58.82%), 2 cases of adenoid cystic carcinoma (11.76%), 1 cases of clear cell carcinoma(5.88%), 1 cases of mucoepidermoid tumor(5.88%), and 7 cases of palate(41.18%).
The results suggested that HPV is not associated to salivary gland tumors, but EBV has strong relation to the induction of the salivary gland tumors.
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